Marble Burying Test In Mice

The marble burying test is a useful model of both obsessive compulsive like ocd behavior and or anxiety like behavior.

Marble burying test in mice. The marble burying test takes advantage of the proclivity of mice to dig in natural settings e g. Burrows escape tunnels and in standard cage bedding and the nestlet shred test capitalizes on the fact that mice are nesting animals by nature that build nests for protection of themselves and their offspring against environmental conditions e. While widely used there is significant controversy over the interpretation of its results. In the test ten marbles are placed evenly spaced on top of the sawdust looking rather like the top of a bourbon biscuit.

Mice with ocd like symptoms tend to engage in a high degree of repetitive behaviors including digging. The marble burying test is used to record the number of marbles buried by mice placed in a novel environment. It has been shown to be sensitive to benzodiazepines as these compounds reduce burying behaviour when. Gyertyan i analysis of the marble burying response.

Eur j pharmacol 2006. If a marble is placed on the surface of the sawdust the mice will naturally play with and bury the marble. When put in a cage with marbles mice with ocd like symptoms tend to engage in a high degree of repetitive behaviors including digging while mice with a high degree of anxiety tend to engage in a high degree of digging in. The marble burying task can be used both as an indicator of obsessive compulsive like ocd behavior and or anxiety like behavior.

Marble burying is an animal model used in scientific research to depict anxiety or obsessive compulsive disorder ocd behavior. This test has some predictive value for anti depressant and or anxiolytic drugs. Mice which are placed individually in a cage bury glass marbles that are present in the cage. Hence we used the marble burying test to examine if acute immobilization stress leads to enhanced anxiety like behavior in c57bl 6 mice if the test is employed with a significant time delay.

Marbles serve to measure digging. A practical screening test with sensitivity to different classes of anxiolytics and antidepressants. In this unit behavioral tests are described that are relevant to the domains affected in asd. For the repetitive domain the t maze spontaneous alternation test and marble burying test are described.

For the communication domain neonatal ultrasonic vocalization and olfactory habituation test toward social and non social odor are described. Usually 30 minutes later at least 8 of the 10 marbles will be. Prinssen a combined marble burying locomotor activity test in mice. We find this test to be sensitive enough to detect the anxiogenic effects even 10 days after a single episode of 2 h immobilization stress.

It is based on the observation that rats and mice will bury either harmful or harmless objects in their bedding.